School of Chemistry

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Adam Nelson

Professor of Chemical Biology

"I am interested in synthetic organic chemistry and its application to biological problems. We focus on the development of new strategies and methods for asymmetric and stereoselective synthesis, which we apply in the synthesis of biologically active molecules and natural products."
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Facilities

Our facilities (many funded through JIF and SRIF initiatives) include a suite of four 500 MHz NMR machines, a state-of-the-art CCD-based X-ray diffractometer, a Nova NanoSEM450 scanning electron microscope, femtosecond laser systems, a category 2 microbiological suite and an automated robotic synthesis and purification laboratory.

Nova NanoSEM450 Scanning Electron Microscope

This instrument operates with a FEG source in standard Secondary Electron and Backscattered Electron modes and also has a CBS detector which makes it possible to image non-conductive samples without coating.

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Scanning Electron Microscope

Joseph Priestley Laboratory

The Joseph Priestley laboratory provides students with the opportunity to perform inorganic and organic chemistry practical work in an industry standard environment and reflects the research-led approach to learning and teaching within the School. It has 63 fume cupboards, with 52 being used for students on a regular basis, and the remaining 11 used for permanent apparatus, and overnight reactions. It can accommodate a maximum of 104 students at any one time.

For further information contact:
Dr Stephen Gorman
Phone 0113 3436512
S.A.Gorman@leeds.ac.uk

Joseph Priestley laboratory

Colour Science Analytical

A range of sophisticated analytical equipment used by postgraduate students and research staff but also available for external contract work. A new scanning electron microscope has recently been installed, providing the capability required to visually examine a wide range of materials and assess their performance and quality.

For further information contact:
Mr Algy Kazlauciunas
Phone 0113 343 2939
A.Kazlauciunas@leeds.ac.uk

Colour science analytical

iPRD Laboratory

Opened in 2009 following an investment of £4.3 million, this laboratory provides facilities for chemical processing and small scale manufacture of chemicals, to order, which allows the inventiveness and creativity of people in academia to be harnessed to provide solutions for chemicals manufacturing companies.

For further information contact:
Prof John Blacker
Phone 0113 343 8239
J.Blacker@leeds.ac.uk

iPRD

NMR

4 x 500 MHz machines offering variable temperature, multinuclear, multidimensional, and diffusion measurement capabilities.

1 x 400MHz machine with solids capability (no low temperature work)

2 x 300MHz machines with 1H/13C and 1H/19F/13C/31P capability and variable temperature, multidimensional capabilities.

NMR

HIRAC (Highly Instrumented Reactor for Atmospheric Chemistry)

A NERC funded atmospheric simulation chamber developed to bridge the divide between the study of elementary reactions in the lab, and large scale field work. Unlike any other atmospheric simulation chambers HIRAC allows researchers the unique ability to control temperature, pressure and photolysis rates during experiments.

For further information contact:
Prof Paul Seakins
Phone 0113 343 6568
P.W.Seakins@leeds.ac.uk

HIRAC

Mass Spectrometry

The School offers mass spectrometery in both serviced and open access modes:

Bruker MicrOTOF
Accurate MS measurements to within 5ppm using electrospray ionisation

Waters GCT Premiere
Accurate MS measurements to within 5ppm using EI, CI and Field Desorption ionsisation methods

Bruker HCT-Ultra
Walk up MS, MS-MS, LC-MS-(MS) measurements using electrospray ionsisation for both small molecule and biological samples

For further information contact:
Dr Stuart Warriner
Phone 0113 343 6437
S.L.Warriner@leeds.ac.uk

Mass Spectrometry

HPLC

The school has a number of HPLC instruments for analytical and preparative work.  A range of chiral columns are also available for ee determination.

2x Agilent 1290 series HPLC systems.  These systems perfrom the majority of our analystical work. The high pressure ratings enable them to be used with rapid resolution columns enabling run time to be shortened to minutes for many applications.

Agilent 1260 Mass Directed Preparative HPLC
Walk up mass directed prep- HPLC system connected to a mass spectrometer – simply specify the mass of your target compound and the system will only collect peaks of the appropriate compound

Gilson Prep HPLC
UV detected Prep HpLC system suitable for flow rates up to 200 ml/min for optimized Preparative HPLC

For further information contact:
Dr Stuart Warriner
Phone 0113 343 6437
S.L.Warriner@leeds.ac.uk

HPLC

Robotic Liquid Handling and Screening

Automation of biological assays is supported through robotic liquid handling and plate reader systems:

Hamilton Star Liquid Handler
An 8 channel robotic liquid handler dispensing volumes between 1ul and 1 ml into a range of different plate formats (eg 96 and 384 well plates). On deck barcode reading, cooling and shaking are all possible. The system is very flexible and is run by a dedicated technician.

Perkin Elmer Envision plate reader: High sensitivity plate reader with large range of filters. Capable of fluorescence intensity, fluorescence polarisation, FRET and TR-FRET measurements along with luminsecnce, and absorbtion measurements in both top and bottom read modes.  In situ injectors allow for rapid kinetic measurements.

Cambridgesoft Information Management system enables results of large compound screens to be automatically processed and compound structure to be linked with results.

For further information contact:
Dr Stuart Warriner 
Phone 0113 343 6437
S.L.Warriner@leeds.ac.uk

Robotic Liquid Handling and Screening

Biological Chemistry Suite

Two category 2 containment labs are fully equipped for molecular biology and protein expression/purification. Facilities include two refrigerated Kuhner shaker-incubators, each with capacity for up to 10 L of bacterial or yeast culture; Constant Systems cell disrupter; Beckman Coulter J30I centrifuge with maximum centrifugal force of 110,000 x g; and Akta Purifier FPLC system.

For further information contact:
Dr Bruce Turnbull
Phone 0113 343 7438
W.B.Turnbull@leeds.ac.uk

Biological Chemistry Suite

X-Ray Diffraction

Bruker X8 single crystal diffractometer facility.
A diffractometer with Apex II area detector and Mo rotating anode source for the determination of single X-ray crystal structures.  We have facilities for either N2 cooling (to 100K) or He cooling (to 30K). Trained users may collect and solve their own data, and we offer two levels of service: data collection only; or data collection with structure solution and refinement.

For further information contact:
Prof Michaele Hardie
Phone 0113 343 6458
M.J.Hardie@leeds.ac.uk

X-Ray Diffraction

Specialist carbon dioxide facilities

Understanding and exploiting the chemistry of carbon dioxide is of vital importance for the future. To enable this, we have a range of equipment for high pressure (liquid and supercritical) and low pressure (gaseous) carbon dioxide, much of it housed in a recently refurbished dedicated interdisciplinary CO2 laboratory funded by the Wolfson Foundation.

For further information contact:
Professor Christopher M. Rayner
Phone 0113 3436579
C.M.Rayner@leeds.ac.uk

Specialist CO2 facilities

Computational methods

Excellent facilities, including a dedicated parallel computing cluster, are available for the in silico design of biological active small molecules. A wide range of projects use the known structure of a target protein as a starting point for the design of small molecule inhibitors. The SPROUT software suite has been continuously developed in Leeds over many years, and allows the de novo design of ligands within the context of a binding site. Researchers are also able to use the computational facilities for bioinformatics provided within the Astbury Centre, for docking, sequence analysis and protein structure prediction.

For further information contact:
Richard Foster
Phone 0113 3435759
R.Foster@leeds.ac.uk

 

 

Specialist CO2 facilities